Electroporation of Postimplantation Mouse Embryos In Utero
نویسندگان
چکیده
منابع مشابه
RNA interference in postimplantation mouse embryos
Sequencing of whole genomes has provided new perspectives into the blueprints of diverse organisms, including the genome of the mouse (Waterston et al., 2002). Although the complete sequence is now available, the estimation of total gene number encoded by the mouse genome is ranging approximately from 25,000 to 50,000 (Okazaki et al., 2002). This uncertainty about the functional units within th...
متن کاملDiffusion MRI of in utero mouse embryos
Introduction The mouse embryo is a widespread animal model for human development disorders. MRI offers a unique, noninvasive approach for imaging normal and abnormal mouse embryo development in later gestational stages. One of the difficulties of imaging in utero mouse embryos is the lack of tissue contrast exhibited by the embryo at high magnet field strengths. This project explores the use of...
متن کاملDevelopmentally regulated gene expression of thrombomodulin in postimplantation mouse embryos.
Embryonic lethality of thrombomodulin-deficient mice has indicated an essential role for this regulator of blood coagulation in murine development. Here, the embryonic expression pattern of thrombomodulin was defined by surveying beta-galactosidase activity in a mouse strain in which the reporter gene was placed under the regulatory control of the endogenous thrombomodulin promoter via homologo...
متن کاملParthenogenetic development of mouse embryos in vivo. II. Postimplantation development.
CBA-p and CBA-T6T6 females were mated with vasectomized males of A strain and early in the morning the eggs were activated in situ with the electric shock of 30, 40 and 50 V. Females were killed between the 5th and the 10th day of pregnancy and the implantation sites were studied histologically or their content was examined under the dissecting microscope. Of the uterine horns, 43-6 % contained...
متن کاملMetabolism of [C]glucose by postimplantation mouse embryos in vitro
Carbon dioxide and lactate production from [C]glucose were measured for postimplantation mouse embryos aged 6 to 9| days post coitum in static cultures with a defined medium. The rate of metabolism increased rapidly and paralleled the increase in protein content indicating a fairly uniform rate of metabolism throughout the period. At all stages studied more than 90 % of the glucose utilized was...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2018
ISSN: 1940-3402,1559-6095
DOI: 10.1101/pdb.prot094060